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Determination of Polysaccharide in Leaves of Morus alba L.

欧Yang-Zhen, Yonghui Li, Shulan Su, Jun Chen

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Abstract

The contents of polysaccharide in leaves of Morus alba L. were measured by anthrone-H2SO4 colorimetry.Wavelength for measurement was 620nm and converting factor of polysaccharide was 3.07;Good linearity ranged from 10～60μg/ml,r=0.999005;Average recovery rate of polysaccharide was 98.81% with 2.08% of RSD(n=5). The results showedthe highest content of polysaccharide was in leaves of Morus alba L. collected at Oct.; The method used in this paper was simple,accurate and stable. It can be used for determination of the polysaccharide in leaves of Morus alba L..

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What this paper is about

The contents of polysaccharide in leaves of Morus alba L. were measured by anthrone-H2SO4 colorimetry.Wavelength for measurement was 620nm and converting factor of polysaccharide was 3.07;Good linearity ranged from 10～60μg/ml,r=0.999005;Average recovery rate of polysaccharide was 98.81% with 2.08% of RSD(n=5). The results showedthe highest content of polysaccharide was in leaves of Morus alba L. collected at Oct.; The method used in this paper was simple,accurate and stable. It can be used for determination of the polysaccharide in leaves of Morus alba L..

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Available abstract

The contents of polysaccharide in leaves of Morus alba L. were measured by anthrone-H2SO4 colorimetry.Wavelength for measurement was 620nm and converting factor of polysaccharide was 3.07;Good linearity ranged from 10～60μg/ml,r=0.999005;Average recovery rate of polysaccharide was 98.81% with 2.08% of RSD(n=5). The results showedthe highest content of polysaccharide was in leaves of Morus alba L. collected at Oct.; The method used in this paper was simple,accurate and stable. It can be used for determination of the polysaccharide in leaves of Morus alba L..

Key concepts: Polysaccharide, Colorimetry, Chemistry, Recovery rate, Anthrone, Chromatography, Botany, Food science

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