2005•Chinese Journal of DisinfectionRequires access

LABORATORY OBSERVATION ON BIOLOGICAL EFFECT OF ELECTROLYZED OXIDIZING WATER

Lin Jun-ming

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Abstract

The electrolyzed oxidizing water (EOW) is produced by electrolysis in EOW-producing machine. In order to observe its bactericidal efficacy and destructive effect on antigenicity of HBsAg, suspension quantitative method was used to carry out experiment. Results: The stock solution of EOW containing available chlorine 220 mg/L with a 5 min contact time killed 100% of spores of Bacillus subtilis var. niger in suspension and the stock solution with a 20 min contact time destroyed HBsAg in suspension to up to the standard. The killing rates of Escherichia coli and Staphylococcus aureus in suspension exposed to dilute solution of EOW containing available chlorine 55 mg/L for 5 min were 100%. After storing in air-tight container protected from light at room temperature for 20 days, the EOW could maintain a redox potential of 1167 mV, pH 2.24 and available chlorine 107 mg/L. Conclusion: The EOW has good germicidal efficacy and is relatively stable.

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What this paper is about

The electrolyzed oxidizing water (EOW) is produced by electrolysis in EOW-producing machine. In order to observe its bactericidal efficacy and destructive effect on antigenicity of HBsAg, suspension quantitative method was used to carry out experiment. Results: The stock solution of EOW containing available chlorine 220 mg/L with a 5 min contact time killed 100% of spores of Bacillus subtilis var. niger in suspension and the stock solution with a 20 min contact time destroyed HBsAg in suspension to up to the standard. The killing rates of Escherichia coli and Staphylococcus aureus in suspension exposed to dilute solution of EOW containing available chlorine 55 mg/L for 5 min were 100%. After storing in air-tight container protected from light at room temperature for 20 days, the EOW could maintain a redox potential of 1167 mV, pH 2.24 and available chlorine 107 mg/L. Conclusion: The EOW has good germicidal efficacy and is relatively stable.

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Available abstract

The electrolyzed oxidizing water (EOW) is produced by electrolysis in EOW-producing machine. In order to observe its bactericidal efficacy and destructive effect on antigenicity of HBsAg, suspension quantitative method was used to carry out experiment. Results: The stock solution of EOW containing available chlorine 220 mg/L with a 5 min contact time killed 100% of spores of Bacillus subtilis var. niger in suspension and the stock solution with a 20 min contact time destroyed HBsAg in suspension to up to the standard. The killing rates of Escherichia coli and Staphylococcus aureus in suspension exposed to dilute solution of EOW containing available chlorine 55 mg/L for 5 min were 100%. After storing in air-tight container protected from light at room temperature for 20 days, the EOW could maintain a redox potential of 1167 mV, pH 2.24 and available chlorine 107 mg/L. Conclusion: The EOW has good germicidal efficacy and is relatively stable.

Key concepts: Stock solution, Oxidizing agent, Chlorine, Chemistry, Electrolysis, Bacillus subtilis, Suspension (topology), Aqueous suspension

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