Study on germicidal efficacy of the electrolyzed oxidizing water from the instruments produced by different manufacture

Objective To compare the germicidal efficacy of Electrolyzed Oxidizing Water (EOW) from instruments offered by the manufactures.To approach bactericidal factors of EOW and factors influencing the germicidal efficacy. Methods A quantitative suspension test was used for detecting the germicidal efficacy of EOW against Staphy aureus, Escherichia coli and spores of Bacillus subtilis var.niger. This test was also used for detecting the germicidal efficacy of HCl+NaOCl against Starphy aureus and observing the influence of 1% peptone on efficacy of EOW in killing spores of Bacillus subtilis var.niger. Results It was found that EOW produced by every instrument of the manufactures with 30 seconds contact-time could attain a killing rate of 100% for Starphy aureus and E.coli and could destroy the antigenicity of HBsAg. The germicidal efficacy of the NaOCl dissolved in distilled water against Starphy aureus was the same as of EOW. As the suspension of spores of Bacillus subtilis var. niger containing or no containing 1% peptone, EOW with 20 minutes contact time could attain a killing rate of 59.94% or 100% respectively. Conclusion EOW produced by instruments of all the manufactures can rapidly kill or destory the Starphy aureus , Escheichia coli , the antigenicity HBsAg. The main germicidal factor of EOW is available chlorine. The 1% peptone has significant influence on germicidal efficacy of EOW.