2009•Hunan Agricultural SciencesRequires access

Determination of Polyphenol in Carambola by Folin-Ciocalteu Colorimetry

Yong Ding

Abstract

The total polyphenol(TP)content of carambola dregs was analyzed by Folin-Ciocalteu colorimetry.The optimum conditions were investigated using gallic acid as standard.The results showed that when the concentration of Folin-Ciocalteu reagent were 1∶1 and the concentration of Na2CO3 was 7.5%,reaction 30 min in 25℃,there was a good linearity relationship between absorption value and carambola polyphenol with the concentration from 0～80 μg/ mL in 765 nm.The method was simple,accurate and suggested as an appropriate method to measure the TP content in carambola dregs

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Key concepts: Colorimetry, Gallic acid, Chemistry, Polyphenol, Reagent, Chromatography, Food science, Biochemistry