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Determination of total polyphenol in barley seed extracts by Folin-Ciocalteu colorimetry

Qing Liu

Open publisher page 6 citations

Abstract

The total polyphenol(TP)content of barley extracts was analyzed by Folin-Ciocalteu colorimetry.The optimum conditions were investigated using gallic acid as standard.The results showed that when the concentration of Folin-Ciocalteu reagent and Na2CO3 were 0.2mol/L and 15%,reaction 60min in room temperature,there was a good linearity between the absorbance and gallic acid concentration from 1～9μg/mL in 760nm.The method was simple,accurate and suggested as an appropriate method to measure the TP content in barley extracts.

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What this paper is about

The total polyphenol(TP)content of barley extracts was analyzed by Folin-Ciocalteu colorimetry.The optimum conditions were investigated using gallic acid as standard.The results showed that when the concentration of Folin-Ciocalteu reagent and Na2CO3 were 0.2mol/L and 15%,reaction 60min in room temperature,there was a good linearity between the absorbance and gallic acid concentration from 1～9μg/mL in 760nm.The method was simple,accurate and suggested as an appropriate method to measure the TP content in barley extracts.

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Available abstract

The total polyphenol(TP)content of barley extracts was analyzed by Folin-Ciocalteu colorimetry.The optimum conditions were investigated using gallic acid as standard.The results showed that when the concentration of Folin-Ciocalteu reagent and Na2CO3 were 0.2mol/L and 15%,reaction 60min in room temperature,there was a good linearity between the absorbance and gallic acid concentration from 1～9μg/mL in 760nm.The method was simple,accurate and suggested as an appropriate method to measure the TP content in barley extracts.

Key concepts: Gallic acid, Colorimetry, Chemistry, Absorbance, Polyphenol, Reagent, Chromatography, Colorimetric analysis

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