2010•Zhongguo shucaiRequires access
Studies on polyphenol content determination of luffa by Folin - Ciocalteu colorimetry.
Huang Shu-ping, Tan TaiMing, Changcheng Xu, Tan Jie, Ming Liu, Meng Wang
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Abstract
A quantitative determination method for polyphenol content in luffa was studied by using Folin-Ciocalteu colorimetry with gallic acid as standard.The results showed that 2 mL polyphenol contents from luffa could be well calculated according to their colorimetric absorption at 770 nm by applying 2 mL Folin-Ciocalteu reagent,and 4 mL 10 % Na2CO3 for 90 min at 30 ℃ for colorimetric reaction,and the linear range of standard curve was 2-10 mg·L-1.The polyphenol content in luffa could be determined by this method.This method is very stable with good reappearing property and high accuracy.