Development of SCAR Markers to Authenticate the Hybrid Seed Lots of Shuza No.6( Brassica napus L.)

Five primers, with which polymorphism bands could be amplified between tw o DNA pools of the parents of hybrid rapeseed, Shuza No.6 , were screened out fro m 100 RAPD primers. Then these five primers were used to amplify the total DNAs of F 1 to determine their specificity and stability. Several maternal specific an d paternal specific DNA bands were amplified with GE204 and GE222, in the DNA pools of Shuza No.6 and its parents. respectively In order to convert the RA PD markers into more stable SCAR (Sequence characterized amplified region) markers, the maternal specific marker GE204 500 and paternal specific marker GE22 2 300 w ere cloned and sequenced respectively. When SCAR primers designed according to t he sequences of GE204 500 and GE222 300 were mixed and used to amplify DNAs of Shu za No.6 and its parents, two distinctive bands, one of which was maternal spec ific and the other was paternal specific, appeared in all hybrid plants, but on ly one parent specific band in maternal or paternal plants. The reliability of this result was confirmed by the phenotypes of hybrid seed lots in the f ield.