Study on Recovery of Bone Myocardial Infarction by Intravenously Delivering Mesenchymal Stem Cell Using Magnetic Targeting Material Fe3O4-UA-g-P(UA-co-AA)

Objective To investigate the extent intravenously transplantation of mesenchymal stem cells(MSCs)mediated by magnetic targeting material arrive in the myocardial infarction region and its effects on the recovery of myocardial infarction.Methods Identify the phenotype of the fourth genet of ex vivo expanded MSCs,stain with DAPI after inducing with 10μmol/L 5-aza,then preserve the MSCs for transplantation.28 SD rats were divided into three groups:group A(n=10),delivered MSCs combined with magnetic targeting material for 30 minutes to rats through tail vein,and kept on raising after placing magnets on the corresponding skin region to myocardial infarction area for 30min;group B(n=9),administration MSCs not conjuncted with magnetic targeting material through tail vein;group C(n=9),direct intramyocardial transplantation of MSCs.Two days after transplantation,evaluate the aggregation state of MSCs in the area of myocardial infarction;30d later,estimate the functional and morphological changes in myocardial infarction region.Results We observed that each MSCs had 3-5 molecules of magnetic targeting material attached to its membrane under transmission electron microscope.The homing rates of MSCs respectively were group A 38%,group B 6%,group C 53%.The number of aggregating MSCs of group A and group C was apparently more than that of group B(P0.01).After transplantation,the contraction indices of left ventricle in group A and group C had significant improvement as compared with that of pre-transplantation(LVEF 46%±6% vs.38%±8%,51%±5% vs.35%±4%;LVFS 28%±6% vs.20%±7%,32%±4% vs.20%±5%,P0.05)and administrated cells stained with DAPI could be detected in infarction region under optical microscope.After transplantation,the contraction indices of left ventricle in group B hadn't conspicuous improvement,and the transplanted cells labeled with DAPI could not be identified in infarction region under optical microscope(homing rate of MSCs 38%).There was no statistically difference of results between group A and group C,but in experiment process,there was a high mortality in group C.Conclusion The method that intravenously delivery of MSCs mediated by magnetic targeting material could accumulate much more MSCs in infarction region,reduce infarction size,and effectively improve the cardiac function after infarction.