Experimental Study of Intravenously Injected CXCR4 Transduced Mesenchymal Stem Cell in Myocardial Infarction

Objective:To study the mechanism(s)and curative effect of mesenchymal stem cells(MSCs)with transgene CXCR4-MSCs and to find a simple and effective therapy for the patients with myocardial infarction(MI).Methods:The female SD rats were divided into/as three groups for MI model(including the MSCs,the CXCR4-MSCs,the saline controls)and the sham operation group randomly.All of the MI rat model's left anterior descending coronary(LAD)were ligated for 60 minutes,followed by reperfusion.Sham-operated rats made a passing a suture around the LAD without ligation.24 hours after MI,2.5×106 Dil-labeled MSCs or CXCR4-MSCs or 1ml saline was injected through the tail vein.3 days and 30 days after cells injection,the number of cells homing and differentiation in MI area was examined and MI size was measured.The total collagen content and the ratio of collagen Ⅰ/Ⅲ,also all of the group rat's left ventricular(LV)function were evaluated by echocardiography 30 days after transplantation.Results:A transwell migration assay:the number of CXCR4-MSCs migrating toward SDF-1 was 3.8 fold greater than the number of MSCs,the number of CXCR4 homing in toward the MI region was significantly increased to 2.5 fold more than the MSCs did.The echocardiographic data in concord with the histomorphological appearance that thinning of the anterior wall was reduced and LV function improved significantly in CXCR4-MSCs group as compared to that of MSCs and saline groups.The collagen Ⅰ/Ⅲratio was lower in the CXCR4-MSC-and MSCs-treated rats by75% as compared to the saline-control group.Conclusion:CXCR4-MSCs can enhance the ability of MSCs migrating toward MI region and improve the curative effect of MI by intravenous transplantation of MSCs.