Drug resistance of extended-spectrum β-lactamases-producing Klebsiella pneumoniae and Escherichia coli causing nosocomial infections in tumor patients

OBJECTIVE To study the distribution and drug resistance characteristics of extended-spectrumβ-lactamases(ESBLs)-producing Klebsiella pneumoniae and Escherichia coli in patients with tumor so as to provide basis for the reasonable clinical use of antibiotics and for the prevention and control of nosocomial infections.METHODS According to National Guide to Clinical Laboratory Operation Procedures,the ESBLs-producing K.pneumoniae and E.coli strains were isolated and identified.The antibiotic susceptibility testing was performed by K-B method and the results were assessed according to latest breakpoints of CLSI.RESULTS Among 132strains of K.pneumoniae and E.coli,totally 33strains of ESBLs-producing isolates were isolated with the total detection rate of 25.00%,including 27(39.71%)strains of ESBLs-producing E.coli and 6(9.38%)strains of ESBLs-producing K.pneumoniae,no strains of pandrug-resistant ESBLs-producing E.coli and K.pneumoniae were detected.The sputum ranked the first place among the specimens sources,accounting for 24.24%.The ESBLs-producing E.coli and K.pneumoniae strains have produced severe resistance to commonly used antibiotics,but were highly sensitive to imipenem and meropenem.CONCLUSION The positive rates of ESBLs-producing K.pneumoniaand E.coli in the tumor patients keep a significant upward tendency.It is necessary to dynamically monitor the change of drug resistance,to grasp the indications for the use of antibiotics,and to implement the related prevention and control measures so as to prevent the outbreak and prevalence of the nosocomial infections caused by the ESBLs-producing strains.