2012•Shandong yiyaoRequires access
Effects of neural stem cell modified by BDNF transplantation on the axonal regeneration and function of hind limbs in rats after spinal cord injury
Sun Kui-xing
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Abstract
Objective To investigate the effects of neural stem cell(NSC)modified by brain derived neurophic factor(BDNF) transplantation on the axonal regeneration and function of hind limbs in rats after spinal cord injury(SCI).Methods Total of 120 SCI rats were divided randomly into 4 groups with 30 in each,the Sham groupreceived laminotomy only,the 3 d after SCI group was given 5 μL normal saline by intraspinal injection,the NSC group was given 5 μL cell suspension of NSC(2×105/μL),the NSC-BDNF group was given 5 μL cell suspension of NSC-BDNF.After 1,3,7,14,28 d,the BDNF and NF200 were detected by immunochemistry,hind limb function of the rats was evaluated using BBB open field locomotion rating score.Results The level of BDNF in NSC-BDNF group was significantly higher than that in the other three groups(P0.05,0.01) in time-dependent manner.The level of NF200 in NSC-BDNF group was significantly higher than that in the other three groups(P0.05),and the BBB score was distinctly higher than that in the SCI and NSC group,was lower than that in Sham group(P0.05,0.01) in time-dependent manner.Conclusion Transplantation of NSCs modified by BDNF can survive in the injured site of spinal cord,can promote the axonal reconstruction and recovery of the function of the hind limb in rats after SCI.