Application of automatic capillary electrophoresis in screening for thalassemia

Objective To evaluate the French Sebia Capillarys 2 automatic capillary electrophoresis in screening for thalassemia.Methods From August to December in 2011,292 specimens of blood screening to detect capillary electrophoresis of hemoglobin,serum iron and ferritin by French Sebia Capillarys 2 automatic capillary electrophoresis(mean corpuscular volume less than 80 fL,MCHC26 pg in patients over 8 years old),while for α-and β-thalassemia gene detection.Results(1) Detected 292 cases of clinical specimens,in which there were 36 cases of α-thalassemia phenotype,135 cases of β-thalassemia phenotype,2 cases bothα-and β-thalassemia phenotype.(2) The high-pressure capillary electrophoresis method forα-thalassemia diagnosis sensitivity was 86.11%,the specificity was 98.37%,accuracy was 95.60%,the positive predictive value was 93.94%,the negative predictive value was 96.03%.(3)The high-pressure capillary electrophoresis method for β-thalassemia diagnosis sensitivity was 97.04%,the specificity was 98.35%,accuracy was 97.66%,the positive predictive value was 98.50%,the negative predictive value was 96.75%.The HbA2 value was greater than 4.00%,β-thalassemia gene detection positive rate was up to 100.00%.Conclusion French Sebia Capillarys 2 automatic capillary electrophoresis with high accuracy for zone resolution and quantification of hemoglobin,which provides rapidly diagnostic basis for preliminary screening of hemoglobinopathy(including thalassemia and abnormal hemoglobinopathy).