Effect of Selenium chitosan on wound healing in vivo

Objective To investigate the effects of Selenium chitosan cream on wound healing of superficial second degree burn in rats.Methods A total of 147Kunming rats were randomly and equally divided into Selenium chitosan cream group 、ground substance group and JingWanHong group.Superficial second degree burn covering 10% of total body surface area was produced by immersing in 70℃ water for 6 second.The wounds were respectively covered with Selenium chitosan cream(1ml/cm2),ground substance(1ml/cm2),and JingWanHong(1ml/cm2).The rats were caged separately,and the dressings were changed once daily.The rats wound healing time were observed in all the groups.7 rats in each group were killed and the biopsies of wound tissues were taken on 12 hours and days1,3,5,7,9 after burn for the determination of water contents、hydroxyproline,NO,TNF,blood ALT and Crea.Another 7 rats were selected as control.Results ①The time of wound healing was(12.9±2.9)days in Selenium chitosan cream group,(16.3±2.1)days in ground substance group,and(11.8±2.4)days in JingWanHong group respectively.The time for wound healing in Selenium chitosan cream group was significantly shorter than that in ground substance group(P0.05).②The water content in the wound tissue in Selenium chitosan cream group on days 1,3,5 after burn[(86.3±3.5)%、(77.8±3.9)%、(72.3±2.7)%]was lower than those in ground substance group[(92.8±3.2)%、(84.9±4.2)%、(77.2±2.8)%,(P0.05)].During 7-9 days after burn,all of them returned to the normal level.③The TNF-αlevel in wound tissue in each group reached the peak at 12-24h after burn,decreased thereafter,but it was still obviuosly higher than that of normal controls on 5 days after burn(P0.05).The TNF-α level in wound tissue in Selenium chitosan cream group and JingWanHong group were markly lower than those in ground substance group at 12h and 1、3、5d after burn(P0.05).④The NO content in wound tissue in each group reached the peak at 12h after burn,decreased thereafter,but it was still obviuosly higher than that of normal controls on 9 days after burn(P0.05).The NO content in wound tissue in Selenium chitosan cream group and JingWanHong group were markly lower than those in ground substance group at 12h and 1d after burn(P0.05).⑤The hydroxyproline content in the wound tissue in Selenium chitosan cream group on days 5,7,9 after burn was higher than that in ground substance group(P0.05)and in JingWanHong group(P0.05).Conclusion Selenium chitosan cream can accelerate wound healing by decreasing wound tissue NO and TNF-α release、enhancing collagen synthesis and lessening permeability and edema.