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ISSR Analysis of Germplasm Resources of Bougainvillea spectabilis Willd

Shao‐Hua Wu

Abstract

Sixty-eight bougainvillea samples were analyzed using ISSR technique to study their taxonomic and phylogenetic relationships.Fourteen ISSR primers were used in the ISSR analysis of 68 bougainvillea individuals.A total of 195 ISSR bands were scored,corresponding to the polymorphic bands were 182,and the percentage of polymorphic was 93.3%.According to ISSR-PCR results,a cluster analysis(UPGMA) was used to generate a dendrogram among all 68 individuals.The results showed that genetic similarity among individuals was between 0.50 and 0.97,the average was 0.67.It was indicated that the relationship among all individuals was close and the genetic diversity was poor.According to dendrogram,68 individuals could be divided into 2 groups at genetic similarity 0.64.The A group could be divided into 2 subgroups and B group could be divided into 6 subgroups.This study revealed the genetic relationship and confirmed the genetic distance among 68 bougainvillea individuals using molecular marker technology.

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Key concepts: UPGMA, Dendrogram, Genetic relationship, Genetic diversity, Germplasm, Biology, Genetic similarity, Similarity (geometry)