Genetic Diversities and Phylogenetic Relationships of Bougainvillea Germplasm Resources Based on RAPD and ISSR Markers

It was studied that the genetic diversities and phylogenetic relationships of 48 Bougainvillea germplasm based on RAPD and ISSR markers in this paper.The results were as follows:(1)The polymorphism was identified by 7 RAPD primers and 11 ISSR primers in the germplasm.97 bands were amplified by RAPD,and 140 bands by ISSR,the polymorphic rate of both markers was 100%.(2)The cluster analysis(UPGMA) showed a higher genetic diversity level based on those polymorphic bands amplified with ISSR and RAPD primers.The genetic similarity coefficient ranged from 0.318 8 to 0.955 6 by RAPD,and from 0.349 5 to 0.900 0 by ISSR.(3)48 samples can be clearly distinguished,and their clustering groups were basically consistent by two markers except a little difference between them.These samples generally originated from two germ lines,of one was from B.glabra,and mainly intraspecific origin,while the other was from species including B.spectabilis,B.peruviana,B.×buttiana and B.×spectoglabra,so the germplasms component were more diverse.(4)The genetic similarity of two markers had a significant correlation(r=0.752 3).The resolution of the molecular markers RAPD and ISSR is more reliable than morpho-agronomic characters to identify individual cultivars according to this investigation.