EFFECT OF LOW TEMPERATURE STRESS ON THE ACTIVITIES OF PM H~+-ATPASE IN SPIRULINA( ARTHROSPIRA) PLATENSIS

Objective: To explore the variation of PM H+-ATPase of the Spirulina platensis( S1)from alkaline lake in Erdos Plateau and its relationship with low temperature resistance under low temperatures stress,and comparing them with that of S. platensis( S2) from Chad Lake in Africa at the same time. Methods: After the purification of the plasma membranes of both Spirulina platensis( S1) from alkaline lake in Erdos Plateau and S. platensis( S2) from Chad Lake in Africa with aqueous polymer two-phase partitioning system,the activities of PM H+-ATPase are determined by Mo-Blue-color method. Results: It is discovered that they being exposed right to low temperature or after exercised under low temperature,as temperature dropping and days lasting,the activities of PM H+-ATPase of both S1 and S2rise first,and drop afterward. The stability of PM H+-ATPase activity of S1 is better than that of S2 at the same conditions above. The activity of PM H+-ATPase can be improved through low temperature exercise. The altering range of PM H+-ATPase activities is S1 S2. They exposed right to low temperatures,the critical low temperature of the physiological activity of S1 is 0℃,S210℃,while it is0℃ for S1,5℃for S2 after exercised under low temperatures. Conclusion: S1 has better adaptability to low temperature.