IGF-1 and PKC expressions in left ventricular remodeling in rats after myocardial infarction

Objective To explore the expressions of cardiac protein kinase C(PKC),IGF-1(insulin-like growth factor 1) and the effect of drugs in the process of left ventricular remodeling(LVRM).Methods SD rats were randomly divided into four groups: sham operation group,infracted group,Captopril group and Valsartan group.Except for sham operation group,left anterior descending coronary artery was ligated in all rats of the other three groups and the intragastric administration of solvent,100 mg·kg~(-1)·d~(-1) Captopril and 20 mg·kg~(-1)·d~(-1) Valsartan was done on day 3 after operation for 8 weeks,then LVRM was evaluated by pathologic analysis.Expression changes of cardiac AgT_(1)R,PKC,and IGF-1 were detected by immunohistochemistry and computer image analysis.IGF-1 mRNA expression was analyzed by RT-PCR.Results LVW,LVWI and the expressions of AgT_(1)R,PKC,IGF-1 mRNA and proteins were significantly enhanced(P0.05 or 0.01) in infarcted group as compared with those in sham operation group.In Captopril group and Valsartan group,LVW,LVWI and the expressions of AgT_(1)R,PKC,IGF-1 mRNA and proteins were remarkably decreased as compared with those in infracted group(P0.05 or 0.01).Conclusion IGF-1 and PKC play a key role in left ventricular remodeling in rats after myocardial infarction.Captopril and Valsartan may be reversed.