Effect of captopril on the expression of tumor necrosis factor-alpha and matrix metalloproteinases-2 in rats with myocardial infarction

Objective To investigate the effect of captopril on ventricular remodeling and cardiac function and the expression tumor necrosis factor-alpha(TNF-α)and matrix metalloproteinase-2(MMP-2)in rats with myocardial infarction.Methods Myocardial infarction model was established by ligation of the anterior descending coronary artery in rats.After 24 hours of operation,survival rats were randomly divided into model group,model+captopril group(rats were treated with 500 mg·kg-1·d-1 captopril in their drinking water),twelve rats in each group.Six rats were selected randomly without ligation as sham operation group.The sham operation group and model group were treated with water.Six weeks after operation,TNF-α and type Ⅰ and Ⅲ collagen were detected with immunohistochemistry;MMP-2 protein were detected by western-blot;Collagen volume fraction(CVF) was assessed with Van Gieson.Left ventricular haemodynamics changes were recorded with baroceptor.Results Compared with sham operation group,the left ventricular function of rats in model group and model + captopril group were obviously degraded(P0.05);Captopril could significantly improve ventricular function after myocardial infarction(P0.05).CVF and typeⅠ/Ⅲ collagen ratio of non-infarct zone in model group and model + captopril group were significantly higher than those in sham operation group(P0.01);Total heart weight/ body weight(THW/BW) and left ventricular weight / body weight(LVW/BW) obviously increased(P0.01).But the CVF,typeⅠ/Ⅲ collagen ratio,THW/BW and LVW/BW in model+captopril group were significantly lower than those in the model group(P0.05).The expression of TNF-α and MMP-2 in the non-infarcted zone of model group were significantly higher than those in sham operation group(P0.01);But in model + captopril group,the expression of TNF-α and MMP-2 were decresed compared with model group(P0.01).Conclusion Captopril could lessen the left ventricular remodeling after myocardial infarction in rats and improves cardiac function.These effects could be relevant to regulate the expression of MMP-2 and TNF-α.