2011•Journal of Anhui Science and Technology UniversityRequires access
Studies on the Methods to Prevent Explant from Browning in Tissue Culture of Soft-seed Punica granatum L.
Gao Hai-lian
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Abstract
The sterilized time and sub-culture time,medium hardness,absorbing agents were studied for reducing the browning rate of explants in tissue culture of soft-seed Punica granatum L.The results showed that the browning control was best when the explants were sterilized by 0.1% mercury solution for 7-9 min,the explants were transferred into new medium every 10d and the treated explants were cultured on MS with agar(5 g/L)+0.1 mg/L NAA+0.1 mg/L IBA+1.0 mg/L 6-BA+30g/Lsucrose+PVP 2g/L.