ScholarLens Back to search
2012•Guizhou nongye kexueRequires access

Study on Influencing Factors on Browning Rate of Explants in Tissue Culture of Gymnadenia conopsea

Yong Wang

Open publisher page 1 citations

Abstract

The surface disinfactant and sterilized time,active carbon,VC in use,dark treatment time,light intensity and interval time for transferring another bottle were studied to reduce the browning rate of explants in tissue culture of G.conopsea.The results showed that the browning rate could reduce from 93.33% to 48.28% when the explants were sterilized by 0.1% mercury solution for 10 min,the explants were transferred into another bottle every three days and the treated explants were cultured on MS + KT 0.5 mg/L +ZT 0.5 mg/L+NAA 1.0 mg/L+AC 1.0 g/L+VC 2.0 mg/L without light at the early time of tissue culture for 5~10 d,light intensity 2 000 lx was the best.

About this research paper

What this paper is about

The surface disinfactant and sterilized time,active carbon,VC in use,dark treatment time,light intensity and interval time for transferring another bottle were studied to reduce the browning rate of explants in tissue culture of G.conopsea.The results showed that the browning rate could reduce from 93.33% to 48.28% when the explants were sterilized by 0.1% mercury solution for 10 min,the explants were transferred into another bottle every three days and the treated explants were cultured on MS + KT 0.5 mg/L +ZT 0.5 mg/L+NAA 1.0 mg/L+AC 1.0 g/L+VC 2.0 mg/L without light at the early time of tissue culture for 5~10 d,light intensity 2 000 lx was the best.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The surface disinfactant and sterilized time,active carbon,VC in use,dark treatment time,light intensity and interval time for transferring another bottle were studied to reduce the browning rate of explants in tissue culture of G.conopsea.The results showed that the browning rate could reduce from 93.33% to 48.28% when the explants were sterilized by 0.1% mercury solution for 10 min,the explants were transferred into another bottle every three days and the treated explants were cultured on MS + KT 0.5 mg/L +ZT 0.5 mg/L+NAA 1.0 mg/L+AC 1.0 g/L+VC 2.0 mg/L without light at the early time of tissue culture for 5~10 d,light intensity 2 000 lx was the best.

Key concepts: Browning, Explant culture, Tissue culture, Darkness, Light intensity, Bottle, Chemistry, Horticulture

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Study on Influencing Factors on Browning Rate of Explants in Tissue Culture of Gymnadenia conopsea — Research Paper | ScholarLens