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2005•Chinese Journal of Spectroscopy LaboratoryRequires access

Determination and Comparison of the Content of Quercetin in Different Kinds of Houttuynia Cordatas Thunb

Qing Bian

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Abstract

A new HPLC method for the determination of quercetin in Houttuynia cordatas thunb was established.90% EtOH was used as solvent and soxtherm distill machine was used to distill the quercetin.Diamonsil~ TMC_ 18(4.6mm×150mm.5μm) and MeOH-H_2O-HAc(48∶49∶3) was made use of chromatogram colum,mobile phase,respectively.The detection wavelength of quercetin is 254nm.The lineal range of quercetin is 0.00704—0.06336μg and the average recovery rate is 98.3% and RSD is 3.2%.The method is simple,sensitive and reproducible,which can be used for the qulity control of Houttuynia thunb cordata material and preparation.

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What this paper is about

A new HPLC method for the determination of quercetin in Houttuynia cordatas thunb was established.90% EtOH was used as solvent and soxtherm distill machine was used to distill the quercetin.Diamonsil~ TMC_ 18(4.6mm×150mm.5μm) and MeOH-H_2O-HAc(48∶49∶3) was made use of chromatogram colum,mobile phase,respectively.The detection wavelength of quercetin is 254nm.The lineal range of quercetin is 0.00704—0.06336μg and the average recovery rate is 98.3% and RSD is 3.2%.The method is simple,sensitive and reproducible,which can be used for the qulity control of Houttuynia thunb cordata material and preparation.

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Available abstract

A new HPLC method for the determination of quercetin in Houttuynia cordatas thunb was established.90% EtOH was used as solvent and soxtherm distill machine was used to distill the quercetin.Diamonsil~ TMC_ 18(4.6mm×150mm.5μm) and MeOH-H_2O-HAc(48∶49∶3) was made use of chromatogram colum,mobile phase,respectively.The detection wavelength of quercetin is 254nm.The lineal range of quercetin is 0.00704—0.06336μg and the average recovery rate is 98.3% and RSD is 3.2%.The method is simple,sensitive and reproducible,which can be used for the qulity control of Houttuynia thunb cordata material and preparation.

Key concepts: Quercetin, Houttuynia cordata, Chemistry, Chromatography, High-performance liquid chromatography, Recovery rate, Biochemistry, Antioxidant

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Determination and Comparison of the Content of Quercetin in Different Kinds of Houttuynia Cordatas Thunb — Research Paper | ScholarLens