Determination of concentration of berberine hydrochloride in various drugs of compatibility of Coptis chinensis

The relationship between efficacy of chinensis and content of berberine in couplet medicine was studied to find the law of compatibility of chinensis using high performance liquid chromatography( RP- HPLC). The chromatography conditions were as follows: the chromatographic column of Agilent C18( 150 × 5 mm,5 μm),with a mobile phase of acetonitrile- monopotassium phosphate( 0. 02 mol / L)( 35 ∶ 65),at a flow of 1m L / min,the detection wavelength was 345 nm,and the column temperature was at room temperature. The metood showed a good linear relationship of berberine hydrochloride in the range of 0. 001 ~ 0. 25 mg / m L( r = 0. 999 3),the concentration of berberine hydrochloride in various drugs of compatibility of chinensis in the range of 0. 273% ~ 0. 606%,the group of Coptis chinensis + Evodia rutatecarpaand Coptis chinensis + Gentianof the highest content,the group of Coptis chinensis + Phellodendronof the lowest content. The change rules of the content of active ingredients was explored based on the effect providing a new development direction for traditional Chinese medicine to research and development of new drugs.