THE EFFECT OF CALCITONIN GENE RELATED PEPTIDE AND NERVE GROWTH FACTOR ON THE EXPRESSION OF c-jun mRNA AND PROTEIN IN STRIATE CORTEX OF RATS WITH TRANSIENT GLOBAL CEREBRAL ISCHEMIA/REPERFUSION

Objective To study the effects of calcitonin gene related peptide(CGRP) and nerve growth factor(NGF) on the expression of(c-junmRNA) and protein in striate cortex of rats with transient global cerebral ischmia/reperfusion. Methods In situ hybridization,immunohistochemistry and microscope image analysis were used. Results The expression of c-jun mRNA in ischemia/reperfusion(I/R) group was increased as compared with sham group(P0.01).The expression of c-jun mRNA was lower in NGF group than that in I/R group(P0.05),and lower in CGRP group than that in I/R group(P0.05),and markedly lower in NGF and CGRP group than that I/R group(P0.01),and in NGF and CGRP group lower than that in NGF group and CGRP group,respectively(P0.05).The expression of c-Jun protein in I/R group was increased as compared with sham group(P0.01).The expression of c-Jun was lower in NGF group than that in I/R group(P0.05),and lower in CGRP group than that in I/R group(P0.05),and markedly lower in NGF and CGRP group than that in I/R group(P0.01),and lower in NGF and CGRP grop than that in NGF group and CGRP group respectively(P0.05).The expression of c-Jun in I/R group was stronger(3?h) survival after ischemia/reperfusion than 1d survival and 3d survival(P0.05),and stronger in NGF and CGRP group 3h survival after ischemia/reperfusion than 1d survival and 3d survival(P0.05).Conclusion The CGRP and NGF downregulate the expression of(c-jun) mRNA and protein,and combined use of NGF and CGRP can markedly downregulate the expression of(c-jun) mRNA and protein in striate cortex of global cerebral ischemia rats.The present results indicate that CGRP and NGF might have coordinate protective effects on the ischemia neurons in striate cortex.