Cloning and Sequensing of NP Gene of Newcastle Disease Virus F_(48)E_9 Strain

According to published NP protein gene sequence of NDV.We designed and synthesized a pair of primers.A 1598bp fragment was amplified from F 48E 9 strain by means of RT-PCR and cloned into the pMD18-T vector.The recombinant plasmid was proved to be true by enzyme analysis and PCR.In order to further identify the credibility of the gene,the sequence of the cDNA was obtained by sanger′s sequencing technique.Compared the obtained gene sequence of F 48E 9 strain with the corresponding sequence of La Sota strain by DNASIS software,the homology of the nucleotide sequence was 88.2%.As a result,we obtained complete NP gene clone of NDV F 48E 9 strain.