2009•Shipin yanjiu yu kaifaRequires access
ANTIOXIDANT ACTIVITY OF ALKALI-HYDROLYED WHEAT BRAN IN VITRO
Yang Liu-qing
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Abstract
To study the preparation and antioxidant effect of alkali-hydrolyzed wheat bran (AHWB) in vitro. Three kinds of AHWB (AHWBⅠ,AHWBⅡ和 AHWBⅢ) were prepared by alkaline hydrolyzing de-starched and de-proteinated wheat bran,and researched on DPPH free radicals,hydroxyl radicals and superoxide radical scavenging. AHWB three can effectively clear the DPPH free radicals and hydroxyl radicals,AHWB Ⅱ be able to clear the superoxide radical. For DPPH,the three alkaline extract of the IC50 values were 0.19 mg/mL,0.54 mg/mL and 0.39 mg/mL respectively,for the·OH,three alkaline extract of the IC50 values were 1.13 mg/mL,1.88 mg/mL and 1.03 mg/mL respectively,which its effect is similar to that of Vc. For O2-·,the IC50 of AHWB Ⅱ is 5.98 mg /mL. The results showed that AHWB has good anti-oxidant activity,and can be used as a natural antioxidant.