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Study on Antioxidant Properties of Total Flavonoids from Wheat Bran in Vitro

Shan Yu

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Abstract

In this paper,the antioxidant property of total flavonoids from wheat bran was investigated.The flavonoid components in wheat bran were extracted by 75 % ethanol refluxing,at 65 ℃ for 2 h,with the ratio of material to solvent was 1∶30(g/L),then purified by macroporous resin and determined by chemical methods.The antioxidant activity of the total flavonoids from wheat bran were evaluated by 1-diphenyl-2-picrylhydrazyl radical(DPPH.) system,hydroxyl radical(.OH) system and superoxide radical(O2-.) system under the reference of vitamin C.Results showed that the total flavonoids extract from wheat bran reached 81.6 % high purity after purification;it can scavenge the DPPH radical,hydroxyl radical and superoxide radical effectively,and the corresponding IC50values were 33.7,113.9 and 206.6 μg/mL,respectively.

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What this paper is about

In this paper,the antioxidant property of total flavonoids from wheat bran was investigated.The flavonoid components in wheat bran were extracted by 75 % ethanol refluxing,at 65 ℃ for 2 h,with the ratio of material to solvent was 1∶30(g/L),then purified by macroporous resin and determined by chemical methods.The antioxidant activity of the total flavonoids from wheat bran were evaluated by 1-diphenyl-2-picrylhydrazyl radical(DPPH.) system,hydroxyl radical(.OH) system and superoxide radical(O2-.) system under the reference of vitamin C.Results showed that the total flavonoids extract from wheat bran reached 81.6 % high purity after purification;it can scavenge the DPPH radical,hydroxyl radical and superoxide radical effectively,and the corresponding IC50values were 33.7,113.9 and 206.6 μg/mL,respectively.

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Available abstract

In this paper,the antioxidant property of total flavonoids from wheat bran was investigated.The flavonoid components in wheat bran were extracted by 75 % ethanol refluxing,at 65 ℃ for 2 h,with the ratio of material to solvent was 1∶30(g/L),then purified by macroporous resin and determined by chemical methods.The antioxidant activity of the total flavonoids from wheat bran were evaluated by 1-diphenyl-2-picrylhydrazyl radical(DPPH.) system,hydroxyl radical(.OH) system and superoxide radical(O2-.) system under the reference of vitamin C.Results showed that the total flavonoids extract from wheat bran reached 81.6 % high purity after purification;it can scavenge the DPPH radical,hydroxyl radical and superoxide radical effectively,and the corresponding IC50values were 33.7,113.9 and 206.6 μg/mL,respectively.

Key concepts: Bran, DPPH, Chemistry, Antioxidant, Hydroxyl radical, Flavonoid, Food science, Superoxide

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