Comparison and Study on the Antioxidant Activity of Pomegranate Peel Extract

Antioxidative activity of Pomegranate Peel extract in vitro was studied.The effect of free radicals scavenging was investigated with different special chemical systems of hydroxyl radical(·OH),superoxide radicals(·O2-),1,1-dipheny-2-picryhydrazyl free radical(DPPH·) radical and inhibitor lipid-peroxidation with homogenates of mouse liver.Ability of Pomegranate Peel extract for half-inhibition of hydroxyl radical and superoxide radicals and DPPH· radicals was as follows:IC50=71.37 mg/mL,SC50=557.55 mg/mL,DC50=31.98 mg/mL,respectively.Half-inhibition of gallic acid was :IC50=51.85 mg/mL,SC50=289.2 mg/mL,DC50=37.65 mg/mL,respectively.Half-inhibition of green tea polyphenols was: IC50=66.67 mg/mL,SC50=1 043.91 mg/mL,DC50=22.48 mg/mL,Inhibitive effects of Pomegranate Peel extract on lipid-peroxidation of homogenates of mouse liver EC50 was 5.21 mg/mL,Gallic acid EC50 was 22.82 mg/mL,Green tea polyphenols EC50 was 3.47 mg/mL,respectively.Pomegranate Peel extract had a good inhibition against lipid-peroxidation.A linear relationship existed between free radicals scavenging ability and inhibitive effects on lipid-peroxidation with treatment of Pomegranate Peel extract.The extract of Pomegranate Peel can effectively scavenge free radical and inhibit lipid-peroxidation significantly.