Effects of Shugan Jiangu Decoction on Osteoblast-Like Cells Proliferation,Differentiation and Evaluation of Estrogenic Potential of Decoction

Objective: To investigate the effects of Shugan Jiangu decoction on osteoblast-like cells and evaluate the estrogenic potential of the decoction.Method: Human osteoblast-like cells MG-63 were cultured and treated with Shugan Jiangu decoction.Cell proliferation was investigated by MTT assay.Alkaline phosphatase activity,as indicator of cell differentiation,was assayed by p-nitrophenylphosphate(pNPP) method.After transient cotransfection with plasmid pERE-TK-Luc and pRL-TK in MCF-7 cells,we evaluated the estrogenic potential of Shugan Jiangu decoction by dual-luciferase reporter assay(DLR).Result: By MTT assay,at 48,72 h,Shugan Jiangu decoction(0.75-12 g.L-1) increased osteoblast-like cell proliferation dose-dependently.By pNPP method assay,at 24,48,72 h,the decoction enhanced alkaline phosphatase activity dose-dependently.By DLR assay,there was no significant difference in estrogenic potential between Shugan Jiangu decoction and negative control.Conclusion: Shugan Jiangu decoction stimulated osteoblast-like cells proliferation and differentiation.No estrogenic potential was observed for Shugan Jiangu decoction.