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2011•The Chinese Journal of Modern Applied PharmacyRequires access

Determination of Luteolin in Celery Seed Extract by HPLC

Gao Yong-liang

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Abstract

OBJECTIVE To establish a method for the determination of luteolin in celery seed extract. METHODS The analysis was performed by HPLC with Apollo C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was consisted of acetonitrile-0.1% phosphoric acid (30 ∶7 0), and the detection wavelength was set at 269 nm. The column temperature was 30 ℃. RESULTS The linear range was 0.50 to 10.0 μg.mL-1 for luteolin (r=0.999 8). The average recovery of three concentration were 100.2%, 100.1% and 101.2%, RSD were 1.02%, 1.91% and 1.75%. CONCLUSION The method is feasible, accurate and high selectivity for the determination of luteolin in celery seed extract.

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What this paper is about

OBJECTIVE To establish a method for the determination of luteolin in celery seed extract. METHODS The analysis was performed by HPLC with Apollo C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was consisted of acetonitrile-0.1% phosphoric acid (30 ∶7 0), and the detection wavelength was set at 269 nm. The column temperature was 30 ℃. RESULTS The linear range was 0.50 to 10.0 μg.mL-1 for luteolin (r=0.999 8). The average recovery of three concentration were 100.2%, 100.1% and 101.2%, RSD were 1.02%, 1.91% and 1.75%. CONCLUSION The method is feasible, accurate and high selectivity for the determination of luteolin in celery seed extract.

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Available abstract

OBJECTIVE To establish a method for the determination of luteolin in celery seed extract. METHODS The analysis was performed by HPLC with Apollo C18 column (4.6 mm×250 mm, 5 μm). The mobile phase was consisted of acetonitrile-0.1% phosphoric acid (30 ∶7 0), and the detection wavelength was set at 269 nm. The column temperature was 30 ℃. RESULTS The linear range was 0.50 to 10.0 μg.mL-1 for luteolin (r=0.999 8). The average recovery of three concentration were 100.2%, 100.1% and 101.2%, RSD were 1.02%, 1.91% and 1.75%. CONCLUSION The method is feasible, accurate and high selectivity for the determination of luteolin in celery seed extract.

Key concepts: Luteolin, Chromatography, Phosphoric acid, Chemistry, High-performance liquid chromatography, Linear range, Flavonoid, Detection limit

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