Determination of Luteolin from Extract of Bamboo Leaves by HPLC

[Objective]The study aimed to establish the method for determining the luteolin content from the extract of bamboo leaves by HPLC.[Method]HPLC was used to determine the luteolin content from the extract of bamboo leaves and its chromatographic conditions were as follows: the chromatographic column was YMC-ODS-A(4.6 mm×250.0 mm,5 μm),the flowing phase was methanol∶0.5% phosphatic acid,with the flow speed of 1.0 ml/min,column temperature of 25 ℃ by linear gradient elution,sample-inject volume of 10 ul and detection wavelength of 335nm.The detected luteolin content was made for the investigation of system suitability and specificity,linear relationship and the precision and also for the tests of stability and adding recovery.[Result]The sample was separated according to the above conditions of chromatography,the luteolin obtained had the retention time of about 38 min,and the sample at 38 min also had the obvious peak,showing that the adaptability and specificity of chromatographic system were good.The luteolin had good linear relationship(R2 = 0.999 6) with the peak area in 0.2~14.0 μg/ml range,with the average recovery of 112.74% and RSD of 2.45%.[Conclusion]The method used for the determination of luteolin was simple and convenient.