ScholarLens Back to search
2013•Zhongguo yaofangRequires access

Content Determination of Luteolin and Apigenin in Ligustrum lucidum by HPLC

Fan Yong-chu

Open publisher page 0 citations

Abstract

OBJECTIVE:To establish the method for the content determination of luteolin and apigenin in Ligustrum lucidum.METHODS:HPLC method was adopted.ZORBAX SB-C18(150 mm×4.6 mm,5 μm)column was used with methanol-0.1% formic acid(55 ∶45,V/V)as mobile phase at the flow rate of 1.0 ml/min.The column temperature was set at 30 ℃ and detection wavelength was 345 nm.RESULTS:The linear range of luteolin and apigenin were 0.040 8-0.652 8 μg(r=0.999 9)and 0.049 2-0.787 2 μg(r=0.999 8),respectively.RSDs of precision,reproducibility and stability tests were all lower than 2%.The average recoveries were 100.6%(RSD=1.04%,n=6)and 100.1%(RSD=1.10%,n=6).CONCLUSIONS:The method is accurate,reliable,sensitive and reproducible,and it is suitable for the quality evalution of L.lucidum.

About this research paper

What this paper is about

OBJECTIVE:To establish the method for the content determination of luteolin and apigenin in Ligustrum lucidum.METHODS:HPLC method was adopted.ZORBAX SB-C18(150 mm×4.6 mm,5 μm)column was used with methanol-0.1% formic acid(55 ∶45,V/V)as mobile phase at the flow rate of 1.0 ml/min.The column temperature was set at 30 ℃ and detection wavelength was 345 nm.RESULTS:The linear range of luteolin and apigenin were 0.040 8-0.652 8 μg(r=0.999 9)and 0.049 2-0.787 2 μg(r=0.999 8),respectively.RSDs of precision,reproducibility and stability tests were all lower than 2%.The average recoveries were 100.6%(RSD=1.04%,n=6)and 100.1%(RSD=1.10%,n=6).CONCLUSIONS:The method is accurate,reliable,sensitive and reproducible,and it is suitable for the quality evalution of L.lucidum.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE:To establish the method for the content determination of luteolin and apigenin in Ligustrum lucidum.METHODS:HPLC method was adopted.ZORBAX SB-C18(150 mm×4.6 mm,5 μm)column was used with methanol-0.1% formic acid(55 ∶45,V/V)as mobile phase at the flow rate of 1.0 ml/min.The column temperature was set at 30 ℃ and detection wavelength was 345 nm.RESULTS:The linear range of luteolin and apigenin were 0.040 8-0.652 8 μg(r=0.999 9)and 0.049 2-0.787 2 μg(r=0.999 8),respectively.RSDs of precision,reproducibility and stability tests were all lower than 2%.The average recoveries were 100.6%(RSD=1.04%,n=6)and 100.1%(RSD=1.10%,n=6).CONCLUSIONS:The method is accurate,reliable,sensitive and reproducible,and it is suitable for the quality evalution of L.lucidum.

Key concepts: Luteolin, Apigenin, Chromatography, Chemistry, Content determination, Formic acid, Reproducibility, High-performance liquid chromatography

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Content Determination of Luteolin and Apigenin in Ligustrum lucidum by HPLC — Research Paper | ScholarLens