Morphological analysis of bone marrow cells with AML1/ETO+AML

Objective To investigate the morphological character of AML1/ETO+ acute myeloid leukemia (AML).Methods The bone marrow smears prepared from 66 AML which detected AML1/ETO fusion gene by fluorescence in situ hybridization(FISH) were stained with Wright′s dye and analyzed according to AML classification standards.Results The cases of morphological analysis of bone marrow cells were diagnosed M2a,M2b,M5,M1,MDS-RAEB-2 which were 52,8,2,2,2,respectively.Among 66 cases of AML,the percentages of AML1/ETO fusion gene positive result was 34.84%.the percentages of positive results with M2a,M2b,M5,M1,MDS-RAEB-2 were 26.9%,100%,0%,0%,50%,respectively.Among the bone marrow smears with AML1/ETO fusion gene,we had detected some abnormal neurtrophilic myelocyte in reviewing analysis.Among 23 with AML1/ETO fusion gene,During treatment,complete remission rate was 78.4%(9 cases of M2a,8 cases of M2b and one cases of RAEB-1) after the first chemotherapy ,4 cases of M2a had complete remission after second chemotherapy.But only 1 case of M2a had no remission(NR) after fifth chemotherapy.Conclusion The results positive for AML1/ETO fusion gene can be detected in all AML-M2b.Some abnormal neurtrophilic myelocyte are found in the bone marrow smears that prepared from AML with positive for AML1/ETO fusion gene.