Simultaneous determination of five components in Shuanghuanglian Powder for Injection by HPLC

AIM:To establish a HPLC method for simultaneously determining chlorogenic acid,caffeic acid,forsythin,forsythoside A and baicalin in Shuanghuanglian Powder for Injection(Flos Lonicerae japonicae,Fructus Forsythiae,Radix Scutellariae,etc.).METHODS:HPLC assay was carried out on a Phenomenex Luna C_ 18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.2% phosphoric acid solution as gradient elution.The flow rate was 1.0 mL/min.The detection wavelength was set as multi-wavelength detection.RESULTS:The linear ranges of chlorogenic acid,caffeic acid,forsythin,forsythoside A and baicalin fell within the range of 0.204-2.04 μg、0.010 2-0.102 μg、0.186-1.86 μg、0.058 5-0.585 μg and 2.52-25.2 μg,respectively.The five components showed good linear correlations(r=0.999 7、0.999 4、0.999 8、0.999 8、0.999 9).The average recoveries of five components were in accordance with the determination requirements.CONCLUSION:The method is simple,quick,reliable,accurate and very suitable to the quality control of Shuanghuanglian Powder for Injection.