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2011•Zhongguo shiyan fangjixue zazhiRequires access

Determination Content of Hyperin in Hypericum attenuatum Choisy

Wei Zuo-gan

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Abstract

Objective:To establish the determination method of hyperin content in Hypericum attenuatum Choisy.Method:VP-ODS Cl8(4.6 mm × 150 mm,5 μm)colmnn was used,the mobile phase was acetonitrile-0.1% phosphoric acid water(16∶ 84);with the flow rate as 1.0 mL.min-1,the detection wavelength was at 360 nm.Result:The linear relation of hyperin was good in the range from 0.099 8 to 0.998 0 μg.The average recovery was 100.11%,RSD 1.20%.Conclusion:The method established is accurate,sensitive and good in reproducibility.It can be applied to the qualitycontrol of H.attenuatum

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What this paper is about

Objective:To establish the determination method of hyperin content in Hypericum attenuatum Choisy.Method:VP-ODS Cl8(4.6 mm × 150 mm,5 μm)colmnn was used,the mobile phase was acetonitrile-0.1% phosphoric acid water(16∶ 84);with the flow rate as 1.0 mL.min-1,the detection wavelength was at 360 nm.Result:The linear relation of hyperin was good in the range from 0.099 8 to 0.998 0 μg.The average recovery was 100.11%,RSD 1.20%.Conclusion:The method established is accurate,sensitive and good in reproducibility.It can be applied to the qualitycontrol of H.attenuatum

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Available abstract

Objective:To establish the determination method of hyperin content in Hypericum attenuatum Choisy.Method:VP-ODS Cl8(4.6 mm × 150 mm,5 μm)colmnn was used,the mobile phase was acetonitrile-0.1% phosphoric acid water(16∶ 84);with the flow rate as 1.0 mL.min-1,the detection wavelength was at 360 nm.Result:The linear relation of hyperin was good in the range from 0.099 8 to 0.998 0 μg.The average recovery was 100.11%,RSD 1.20%.Conclusion:The method established is accurate,sensitive and good in reproducibility.It can be applied to the qualitycontrol of H.attenuatum

Key concepts: Chromatography, Phosphoric acid, Chemistry, Reproducibility, Recovery rate, Linear range, Hypericum, Botany

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