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HPLC Determination of Hyperin in Compound Hypericum Perforatum Dropping Pills

Xing Jian-guo

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Abstract

Objective: To establish the method to determine the concentration of hyperin in Compound Hypericum Perforatum Dropping Pills.Method: HPLC was used for the determination of hyperin in Compound Hypericum Perforatum.A COSMOSIL packed column 5 C18-MS-Ⅱ(4.6 mm × 250 mm,5 μm) was used.The mobile phase was acetonitrile-water(0.5% glacial acetic acid and 2% tetrahydrofuran)(13∶87).The detection wavelength was set at 360 nm.Result: The linear range for hyperin was 10.8-54.0 mg.L-1(r =0.999 1).The average recovery was 98.31%(RSD 1.23%).Conclusion: The method can control the quality of Compound Hypericum Perforatum Dropping Pills effectively.It was sensitive,accurate and feasible.

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What this paper is about

Objective: To establish the method to determine the concentration of hyperin in Compound Hypericum Perforatum Dropping Pills.Method: HPLC was used for the determination of hyperin in Compound Hypericum Perforatum.A COSMOSIL packed column 5 C18-MS-Ⅱ(4.6 mm × 250 mm,5 μm) was used.The mobile phase was acetonitrile-water(0.5% glacial acetic acid and 2% tetrahydrofuran)(13∶87).The detection wavelength was set at 360 nm.Result: The linear range for hyperin was 10.8-54.0 mg.L-1(r =0.999 1).The average recovery was 98.31%(RSD 1.23%).Conclusion: The method can control the quality of Compound Hypericum Perforatum Dropping Pills effectively.It was sensitive,accurate and feasible.

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Available abstract

Objective: To establish the method to determine the concentration of hyperin in Compound Hypericum Perforatum Dropping Pills.Method: HPLC was used for the determination of hyperin in Compound Hypericum Perforatum.A COSMOSIL packed column 5 C18-MS-Ⅱ(4.6 mm × 250 mm,5 μm) was used.The mobile phase was acetonitrile-water(0.5% glacial acetic acid and 2% tetrahydrofuran)(13∶87).The detection wavelength was set at 360 nm.Result: The linear range for hyperin was 10.8-54.0 mg.L-1(r =0.999 1).The average recovery was 98.31%(RSD 1.23%).Conclusion: The method can control the quality of Compound Hypericum Perforatum Dropping Pills effectively.It was sensitive,accurate and feasible.

Key concepts: Hypericum perforatum, Chromatography, High-performance liquid chromatography, Acetic acid, Chemistry, Hypericum, Traditional medicine, Medicine

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