Effects of ginsenoside Rb1 on cell proliferation,differentiation and OPG/RANKL mRNA expression of rat osteoblast cultured in vitro

Objective:To study the effects of ginsenoside Rb1 on cell proliferation,differentiation and OPG/RANKL mRNA expression of rat osteoblast cultured in vitro.Methods:Rat osteoblast cultured in vitro was treated by a series of different concentration of ginsenoside Rb1 for different time respectively.The proliferation of osteoblast was detected by MTT method.The differentiation of osteoblast was detected by the activity of ALP.mRNA expression of osteoprotegerin (OPG) and RANKL(receptor activator nuclear factor-kappa B ligand,RANK) were semi-quantified by RT-PCR,and the ratio of OPG/RANKL mRNA was used to analyze the differentiation of ginsenoside Rb1 to osteoclast.Results:Ginsenoside Rb1 at a concentration of 1.12×10-9mol/L to 1.12×10-5mol/L can promote the proliferation of osteoblast after 72h of treatment (P0.01);1.12×10-7mol/L and 1.12×10-6mol/L can enhance the differentiation of osteoblat;1.12×10-9mol/L and 1.12×10-8mol/L can raise the ratio of OPG/RANKL mRNA of rat osteoblast cultured in vitro and inhibit the differentiation of osteoclast (P0.01),1.12×10-7mol/L and 1.12×10-5mol/L can make the ratio of OPG/RANKL mRNA down regulation (P0.01).Conclusion:Ginsenoside Rb1 could influence the proliferation,differentiation of the osteoblast and the formation of the osteoclast markedly.