Establishment And Apply of duplex RT-PCR Assay for Detecting Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus And Porcine Reproductive and Resperatory Syndrome Virus

Based on the mutation characteristic of genome sequence of highly pathogenic porcine reproductive and respiratory syndrome virus(HP -PRRSV), and low pathogenic porcine reproductive and respiratory syndrome virus (LP-PRRSV),two pair primer s were designed,a rapid duplex RT -PCR method for detecting HP-PRRSVand LP-PRRSV were developed.1498 samples were detected with the eatablished duplex RT-PCR assay.Compared with commercial kits,the results indicated sensitivity high and specificity good.And the methods avoid the risk of time,reagent consuming, the pollution.the assay laid solid foundation for PRRSV detection and epidemiology.