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2008•Journal of the South China Agricultural UniversityRequires access

Diagnosis and Identification of the Highly Virulent Porcine Reproductive and Respiratory Syndrome Virus by RT-PCR

Dongsheng He

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Abstract

A pair of primers based on Nsp2 gene of porcine reproductive and respiratory syndrome virus(PRRSV) was designed and a method of RT-PCR was developed to distinguish the highly pathogenic PRRSV from the classic attenuated vaccine strain.The method was sensitive to detect as low as 0.2 ng viral genome.PCV2,PRV,PPV and CSFV were negative in their reactions.

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What this paper is about

A pair of primers based on Nsp2 gene of porcine reproductive and respiratory syndrome virus(PRRSV) was designed and a method of RT-PCR was developed to distinguish the highly pathogenic PRRSV from the classic attenuated vaccine strain.The method was sensitive to detect as low as 0.2 ng viral genome.PCV2,PRV,PPV and CSFV were negative in their reactions.

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Available abstract

A pair of primers based on Nsp2 gene of porcine reproductive and respiratory syndrome virus(PRRSV) was designed and a method of RT-PCR was developed to distinguish the highly pathogenic PRRSV from the classic attenuated vaccine strain.The method was sensitive to detect as low as 0.2 ng viral genome.PCV2,PRV,PPV and CSFV were negative in their reactions.

Key concepts: Porcine reproductive and respiratory syndrome virus, Virology, Virulence, Virus, Biology, Highly pathogenic, Gene, Polymerase chain reaction

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