Determination of nucleotides in cordyceps by RP-HPLC

A reversed-phase high performance liquid chromatography was developed for the determination of nucleotides (uridine,guanosine,adenosine and adenine)in cordyceps. The nucleotides were well separated and determined on an Alltima C18 column (250mm×4.6mm, 5μm)by using a mixture of acetonitrile-water (V/V=4:96) at 260nm with the flow rate of 1.0ml/min. The results showed that the developed method was sensitive, accurate, reproducible and high recovery(98.7%～101.5%) and suitable for analysis of nucleotides in cordyceps. The contents of uridine,guanosine,adenosine and adenine were ~10.52 mg/g,~8.56 mg/g,~0.708 mg/g and ~1.79 mg/g, respectively, with the average RSD ranged between 0.51% and 1.30% (n=5).