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2012•Journal of Nuclear Agricultural SciencesRequires access

SYNTHESIS AND IDENTIFICATION OF THE ARTIFICIAL ANTIGEN FOR DiHT IN IRRADIATION FOOD

Lei Wang

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Abstract

In the present study,5,6-Dihydrothymidine Hapten(DiHT-COOH) was synthesized by using 5,6-Dihydrothymidine(DiHT) as raw material.DiHT-COOH was identified by thin layer chromatography and 1H-NMR.Hapten was conjugated to bovine serum albumin(BSA) and ovalbumin(OVA) to obtain artificial immune antigen and coating antigen,respectively.After detected by UV spectroscopy and SDS-PAGE,the ratios of Hapten to protein were calculated.The conjugation ratios of immunogen and coating antigen were 9.8∶1 and 12∶1,respectively.Antisera were obtained by immunizing BALB/c with immunogen and the titer were 1∶2.56×104,which indicated the synthesis of artificial antigen was successful.

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What this paper is about

In the present study,5,6-Dihydrothymidine Hapten(DiHT-COOH) was synthesized by using 5,6-Dihydrothymidine(DiHT) as raw material.DiHT-COOH was identified by thin layer chromatography and 1H-NMR.Hapten was conjugated to bovine serum albumin(BSA) and ovalbumin(OVA) to obtain artificial immune antigen and coating antigen,respectively.After detected by UV spectroscopy and SDS-PAGE,the ratios of Hapten to protein were calculated.The conjugation ratios of immunogen and coating antigen were 9.8∶1 and 12∶1,respectively.Antisera were obtained by immunizing BALB/c with immunogen and the titer were 1∶2.56×104,which indicated the synthesis of artificial antigen was successful.

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Available abstract

In the present study,5,6-Dihydrothymidine Hapten(DiHT-COOH) was synthesized by using 5,6-Dihydrothymidine(DiHT) as raw material.DiHT-COOH was identified by thin layer chromatography and 1H-NMR.Hapten was conjugated to bovine serum albumin(BSA) and ovalbumin(OVA) to obtain artificial immune antigen and coating antigen,respectively.After detected by UV spectroscopy and SDS-PAGE,the ratios of Hapten to protein were calculated.The conjugation ratios of immunogen and coating antigen were 9.8∶1 and 12∶1,respectively.Antisera were obtained by immunizing BALB/c with immunogen and the titer were 1∶2.56×104,which indicated the synthesis of artificial antigen was successful.

Key concepts: Immunogen, Hapten, Ovalbumin, Antigen, Bovine serum albumin, Antiserum, Titer, Chemistry

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