Synthesis and Identification of Artificial Antigen for Bensulfuron

In this study,a hapten was synthesized from methyl 2-[(sulfamoyl)methyl] benzoate and succinic anhydride.The synthetic hapten was identified by thin layer chromatography and 1H-NMR spectroscopy.An immune antigen(haptenBSA) and a coating antigen(hapten-OVA) were prepared through separate conjugation of the hapten with BSA and OVA by mixed anhydride method.The conjugation ratios of the immune antigen and coating antigen were calculated to be 8:1 and 13:1 based on UV spectroscopic analysis,respectively,suggesting their good immunogenicity.Positive sera with a titer of 1:32000 were obtained by animal immunization trials.The IC50 and IC10 of antibody were determined by indirect ELISA assay to be 0.5032 mg/L and 0.0024 mg/L,respectively.Therefore,the synthesis of artificial antigen is successful.