The anti-tumor effects of CIK and its supernatant against gastric cancer cell lines SGC-7901 in vitro

Objective:To observe the proliferation ability of cytokine-induced kill cells(CIK) and investigate the anti-tumor effects of CIK against gastric cancer cell lines SGC-7901 in vitro.Methods:The peripheral blood mononuclear cells(PBMC) of healthy human were induced with different cytokines,and transferred in CIK cells.The proliferation ability of CIK cells was measured by calculation in different culturing time,and the phenotype of CIK cells was analyzed by a flow cytometer.Inverted microscope to observe morphological changes of SGC-7901 gastric cancer cell after added the CIK cells.The CIK cell culture supernatant induced gastric cancer cells apoptosis was analyzed by a flow cytometer.The antitumor cytotoxicity of CIK cells against SGC-7901 cell lines was measured by MTT assay.Results:With the cultured time of CIK cells prolong,both the number and killing activity increased;CIK cells possessed strong proliferate ability with 111.63±10.97 flod expansion after 21 days culture,CD3+ CD56+ double positive cells in the ratio was up to 35.8±9.7 percents,and then,both the proliferate ability of CIK cells and the percentage of CD3+CD56+ cells declined.The CIK cell supernatant can induce apoptosis of gastric cancer cell.CIK cells possessed significant ability to kill SGC-7901 tumor cells,the maximum killing efficiency is about(74.91±2.71)%.Conclusion:CIK cells own strong anti-cancer activity against SGC-7901 gastric cancer cells in vitro.The suitable time for clinical application is at day 14 to 21,which is the proliferation peak.The main Killing effect was through direct killing and inducing apoptosis.