Research on submerged fermentation process of phaeoporus obliquus

Aim To study the submerged fermentation process and amino acid analysis of Phaeoporus obliquus strain.Methods By adopting L 9(3 4) just cross experimentatiaon method to optimize culture medium.Results The composition of the optimal fermentation medium was as fellows:8%glucose,1.60%Powdery yeast,0.3%KH 2PO 4,0.15% MgSO 4,V B1 10mg/100mL,pH 6.0.The optimum cultural period was 7 day in 100/300 mL shake flasks at 150 r/min.The best aeration ratio was 2:5(medium volume:flask volume).The inoculation volume was 10%.The highest dry weight of mycelia of Phaeoporus obliquus was obtained about 1.350 g/150 mL in the optimum culture.Conclusion It is feasible that the mycelia of Phaeoporus obliquus were made by submerged fermentation process,Phaeoporus obliquus was provided with biological characteristic of limited oxygen.