Effect of the Buqi Huoxue Decoction on Expressions of bcl-2 and bax in Chronic Bronchitis Rats with Deficiency of Lung-qi

Objective To observe the effect of the Buqi huoxue decoction on expressions of bcl-2 and bax in chronic bronchitis( CB) rats with deficiency of lung-qi. Methods Ten rats were randomly selected from 50 SD rats as control group,and models of the deficiency of lung-qi of chronic bronchitis were established using the improved smokingfumigated method in the other 40 rats. The successful model rats were randomly divided into model group,high-dose group of Buqi huoxue decoction,low-dose group of Buqi huoxue decoction and tonifying lung recipe group( n = 10 for each group). Expressions of apoptosis-related gene bcl-2 and bax were detected using immunohistochemistry method. Results Compared with those in control group,the bcl-2 expression in the model group was significantly increased,while the bax expression was significantly decreased( P 0. 01); compared with those in model group,the bcl-2 expressions were significantly reduced,while the bax expressions were significantly increased in high-dose,low-dose and tonifying lung recipe groups( P 0. 01); the bcl-2 expression in high dose group was significantly decreased,while the bax expression in high dose group was significantly increased than those in low dose and tonifying lung recipe groups( P 0. 01); the bcl-2 expression was significantly lower,while the bax expression was significantly higher in low-dose group than that in tonifying lung recipe group( P 0. 05). Conclusion The Buqi huoxue decoction can inhibit the expression of apoptosis-related gene bcl-2,increase the expression of bax in inflammatory cells and promote inflammatory cell apoptosis,which can be used as an effective method of prevention and treatment of chronic bronchitis.