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STUDY ON OPTIMIZING PURIFICATION TECHNOLOGY OF TOTAL FLAVONOIDS FROM PAPER MULBERRY(BROUSSONETIA PAPYRIFERA L.) FRUITS WITH MACROPOROUS RESIN

Yan Li

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Abstract

To find a better macroporous resin for separation and purification of total flavonoids from paper mulberry fruits and establish the optimum technological conditions, eight types of macroporous resin were screened by static adsorption efficiency and desorption efficiency, and the optimum parameters of dynamic adsorption and desorption were studied. D101 resin showed the best performance. The purification conditions were specified as the following: flow velocity of adsorption, pH value and concentration of sample solution were 2BV/h, 6 and 0.05mg/mL,respectively. The eluting reagent, desorption velocity and elution volume were 80% ethanol,5BV/h and 7.5BV, respectively. Under these conditions, the content of total flavonoids was22.26%, which was 4.79 times higher than before. The results showed that the total flavonoids from paper mulberry fruits could be effectively purified by D101 resin.

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What this paper is about

To find a better macroporous resin for separation and purification of total flavonoids from paper mulberry fruits and establish the optimum technological conditions, eight types of macroporous resin were screened by static adsorption efficiency and desorption efficiency, and the optimum parameters of dynamic adsorption and desorption were studied. D101 resin showed the best performance. The purification conditions were specified as the following: flow velocity of adsorption, pH value and concentration of sample solution were 2BV/h, 6 and 0.05mg/mL,respectively. The eluting reagent, desorption velocity and elution volume were 80% ethanol,5BV/h and 7.5BV, respectively. Under these conditions, the content of total flavonoids was22.26%, which was 4.79 times higher than before. The results showed that the total flavonoids from paper mulberry fruits could be effectively purified by D101 resin.

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Available abstract

To find a better macroporous resin for separation and purification of total flavonoids from paper mulberry fruits and establish the optimum technological conditions, eight types of macroporous resin were screened by static adsorption efficiency and desorption efficiency, and the optimum parameters of dynamic adsorption and desorption were studied. D101 resin showed the best performance. The purification conditions were specified as the following: flow velocity of adsorption, pH value and concentration of sample solution were 2BV/h, 6 and 0.05mg/mL,respectively. The eluting reagent, desorption velocity and elution volume were 80% ethanol,5BV/h and 7.5BV, respectively. Under these conditions, the content of total flavonoids was22.26%, which was 4.79 times higher than before. The results showed that the total flavonoids from paper mulberry fruits could be effectively purified by D101 resin.

Key concepts: Chemistry, Desorption, Adsorption, Chromatography, Broussonetia, Reagent, Elution, Volume (thermodynamics)

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STUDY ON OPTIMIZING PURIFICATION TECHNOLOGY OF TOTAL FLAVONOIDS FROM PAPER MULBERRY(BROUSSONETIA PAPYRIFERA L.) FRUITS WITH MACROPOROUS RESIN — Research Paper | ScholarLens