2012•China BrewingRequires access
Optimization of fermentation conditions for erythromycin-producing strain
Huang Sisi
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Abstract
The optimal fermentation conditions of mutant Saccharopolyspora erythraea N5 producing erythromycin were investigated through single factor and orthogonal experiment.The optimal medium compositions were as followed: glucose 3%,starch 3%,corn plasma 0.15%,ammonium sulfate 0.7% and soybean oil 0.3ml/5ml.In addition,the optimal erythromycin fermentation condition were as followed: inoculum 10%,medium volume of shaking flask 50ml/250ml,initial pH value 6.5,the culture temperature 33℃ and fermentation time 156h.