Optimization of Fermentation Medium for Erythromycin Production of Streptomyces erythreus by Response Surface Methodology

Objective To optimize the fermentation medium for erythromycin production Streptomyces erythreus by response surface methodology.Methods Min Run Res Ⅳ design was used to evaluate the effect of compositions of organic nitrogen source and carbon source,and the steepest ascent path was employed to determine the central region of the medium composition for the further central composite design(CCD).Results Starch,dextrin,soybean cake meal and corn steep liquor showed significant effect on the yield of erythromycin,of which the optimal proportion in medium were 5.24%,1.43%,4.56% and 1.47% respectively.Conclusion The carbon and nitrogen sources of fermentation medium for erythromycin production of Streptomyces erythreus were successfully optimized by response surface methodology.The chemical titer of erthyromycin produced by fermentation in shake flask was 6 192 U/ml.