Effect of aisaticoside on apoptosis of PC12 cells induced by Aβ_(25～35)

Objective: To investigate effects of aisaticoside on apoptosis of PC12 cells induced by Aβ25~35.Methods: Aisaticoside at 160,80,40,20 μg·mL-1 were added into the cultures of PC12 cells in the presence of 20 μmol·L-1 Aβ25~35.Cell viability was determined with CCK-8;apoptosis was tested by flow cytometry,cell morphology was observed by fluorescence microscopy,and Bcl-2 expression was detected by Western blotting.Results: Compared to Aβ25~35 alone,aisaticoside increased cell viability and Bcl-2 expression,decreased apoptosis in a concentration-dependent manner.Conclusion: Aisaticoside inhibits PC12 cell apoptosis induced by Aβ25~35,which may be associated with increasing Bcl-2 expression.