Two-step Enzymatic Extraction of Banana Resistant Starch

In this paper,a two-step enzymatic method was used for the extraction of banana resistant starch(RS) by using pectase,cellulose and a-amylase.The results showed that,in the first enzymolysis step,the optimum conditions were mass ratio of pectinase to cellulase of 1:2,enzyme dosage of 0.22%,reaction temperature of 45 ℃,pH value of 5.0 and reaction time of 35min.In the second enzymolysis step,the best extraction conditions were a-amylase dosage of 0.35%,reaction temperature of 52 ℃,pH value of 6.3 and reaction time of 3.5 h.With this method,the extract rates of RS were 81.24%.