Condition Optimization of Acorn Starch Extraction by Alkali Protease Enzymolysis
Guoquan Zhang
Abstract
Guoquan Zhang
Abstract
To optimization the extraction technology of acorn starch,enzymolysis time,pH value,temperature,enzyme dosage of alkalinity protease,were selected to take orthogonal experiment of conditions of starch extraction by alkaline protease enzymolysis.The results showed that,the affecting factors by alkaline protease enzyme treatment were pH value,enzymolysis temperature,enzymolysis time,and enzyme dosage of alkali protease.The optimization of process conditions by enzymolysis using alkali protease is that the pH is 11,the enzymolysis temperature is 45 ℃,the enzymolysis time is 140 minutes,enzyme dosage is 600 U/g,the content of protein in the starch is 2.32%,the west starch obtained after enzymolysis is soda-wash bleached when the mass rate to starch and water is 1:5,the concentration of aquae hydrogen dioxide is 1%,the pH is 11,temperature is 45 ℃ and time is 12 hours,protein residues in the final starch is not examined.
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To optimization the extraction technology of acorn starch,enzymolysis time,pH value,temperature,enzyme dosage of alkalinity protease,were selected to take orthogonal experiment of conditions of starch extraction by alkaline protease enzymolysis.The results showed that,the affecting factors by alkaline protease enzyme treatment were pH value,enzymolysis temperature,enzymolysis time,and enzyme dosage of alkali protease.The optimization of process conditions by enzymolysis using alkali protease is that the pH is 11,the enzymolysis temperature is 45 ℃,the enzymolysis time is 140 minutes,enzyme dosage is 600 U/g,the content of protein in the starch is 2.32%,the west starch obtained after enzymolysis is soda-wash bleached when the mass rate to starch and water is 1:5,the concentration of aquae hydrogen dioxide is 1%,the pH is 11,temperature is 45 ℃ and time is 12 hours,protein residues in the final starch is not examined.
Key concepts: Starch, Chemistry, Alkalinity, Protease, Extraction (chemistry), Chromatography, Food science, Enzyme